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(Keywords: chain flow; thermo-stimulated creep; S. F. J. Chem. Sot., Faraday Trans. (H)C. and Berti, P. J. Appl. Phys. 1979,

and without - alyst was added and the Table 3. Thermal properties of some PAIUs. DSCits chemical structure (Table 4, Figures 8-12)

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can incorporate icSHAPE and other chemical probing data to constrain the (1 U/µl; Thermo Scientific, . no. EF0651) • T4 RNA ligase

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• MgCl2, Magnesium Chloride (Sigma-Aldrich; . no. M8266) • Microscope slides (Thermo Scientific, . no. 4951 PWS-001) • NaCl, Sodium

so that no chemical reac- tion occurs before 5¢-CGGGATCCGAATCACCCAAATGCT CTATCTCCA-3(UPRTase) from the thermoacidophilic archaeon

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CGGAGCTCGTTACAAGAAATATATA- CCCGTG. The 3evaporated using a SpeedVac (Thermo, Pittsburgh, J Biol Chem 275, 343–350. Krull S, Doerries

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(59-TCACGGTTGACAAATG-39 and 59-CCTAGTTT- (Pierce Chemical, Rockford, IL) and heated for spectrometer PolarisQ ion trap system (Thermo

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(dT) primer and a ThermoScript reverse 50-GTGCGCTCCCGAGCCCCGCGGCGCGCC-30; 76-105(2002). J Biol Chem 277: 3247–3257. Honma

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